his experimental study was performed to compare the effects of formocresol, calcium hydroxide and glutaraldehyde on the vitally amputated pulp tissues. 8 premolars of each sample, total of 64 from 8 young sound dogs were pulpotomized with one of the following 8 different methods. i.e., 5 minutes application of formocresol on the amputated pulp surface and capping with zinc oxide eugenol paste mixed with formocresol, 5 minutes application of formocresol and capping with calcium hydroxide mixed with or without formocresol, 4 days application of formocresol and capping with either zinc oxide eugenol paste or calcium hydroxide, direct capping with calcium hydroxide only or calcium hydroxide mixed with formocresol, and 5 minutes application of 2% glutaraldehyde and capping with calcium hydroxide mixed with Vlo´ glutaraldehyde.
3 dogs were sacrificed 3 weeks later and the other 5 were sacrificed 200 days later respectively, and all the pulpotomized teeth were prepared for histological findings. The obtained results were as follows;
1. In the pulp tissues that were treated with formocresol and capped with zinc oxide engenol mixed with or without formocresol, no dentin bridge was observed and inflammatory response seemed to progress deeper leading to necrosis of the pulp sooner or later.
2. In the pulp tissues that were treated with formocresol for 5 minutes and capped with calcium hydroxide mixed with or without formocresol, or directly capped with calcium hydroxide mixed with formocresol, relatively mild inflammatory reaction was observed with complete dentin bridge formation and the vitality of the remaining pulp tissues app-eared to be sligtly degenerated.
3. In the pulp tissues that were treated with formocresol for 4 days, generally degenerative-and destructive change was severer than in the ones that were treated for 5 minutes.
4. In the pulp tissues that were capped with calcium hydroxide, more favorable tissue reaction was observed when used with formocresol than in the ones where formocresol was. not used.
5. In the pulp tissues that were treated with 2% glutaraldehyde and capped with calcium hydroxide mixed with 2% glutaraldehyde instead of formocresol, generally compact dentin bridge was formed and the vitality of the remaining pulp tissues was preserved for a long time relatively with less inflammatory response.
6. In the early stage of osseous structure formation, it appeared relatively spongeous structure showing many spaces, forming tunnels between capping material and the remaining pulp. In the favorable cases, the space was more rapidly closed and dentin bridge was densely added along the lower border of osseous structure covering the remaining pulp, on the other hand, as the formation of dense dentin bridge was retarded, it was more spacious and thus the inflammatory stimulus penetrating deeper and destroying the remaining pulp more severely.
7. The vitality of remaining pulp tissue, pulpotomized with most of the usual methods, seemed to be declined so the author suppose that further more improved method would be necessary.
|